Our Multi-Parameter Toxicity assay’s apply HUREL’s superior metabolic competency to a panel of complementary assays, creating a multi-parametric characterization of hepatotoxic risk. HUREL Tox™ incorporates a 14-day, repeat-dose cytotoxicity study with both ATP and albumin (ELISA) readouts. Computation of time-based HUREL ratios™ and ACEA RTCA impedance signal indices illuminate potential chronic liabilities of pre-candidate NME’s. Incubation in the absence and presence of broad CYP inhibitor aminobenzotriazole (ABT) reads on the potential for formation of reactive metabolites. Incubation in the absence and presence of bile salts provides a marker of potential cholestatic liability.
When provided as a contract research service, the HUREL Tox™ assay panel can be custom-configured according to the client’s particular study design.
Protocol
14-day, repeat-dose cytotoxicity study. Readouts include:
- ATP – 24 hr, 7-day, 14-day
- Acea xCELLigence® real-time impedance monitoring
- +/- Bile Salts
- +/- ABT
- ALT
Models Available
All HUREL micro liver models
Study Turnaround Time
Receipt of sample to data delivery, is 21 days
Example Results
