HUREL® Minipig™

Co-culture comprised of cryopreserved primary minipig hepatocytes cultured with cells of non-parenchymal, stromal type.

Example Metabolic Activity (nmoles/hr/106 cells)

SubstrateEnzymeConcentration (µM)Day 1Day 8
7-EthoxycoumarinPhase I (ECOD)1000.234.239
7-HydroxycoumarinPhase II(UGT)10088.8104.4
7-HydroxycoumarinPhase II (SULT)100.980.836

Culture Condition and Morphology

Cryopreserved minipig hepatocytes were thawed and plated with HUREL PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to HUREL PlatinumHeps™ maintenance Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/million cells. All incubations were carried out in triplicate on days 1 and 8 (customer day) upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Collected supernatants were stored at -20°C until further LC/MS/MS analysis.

Day 1 – Morphology

Day 7 – Morphology

Day 7 – Bile Canaliculi

Mini Pig Day 1

Phase contrast image in a 24-well at a 10x magnification

Mini Pig day 7

Phase contrast image in a 24-well at a 10x magnification

Mini Pig Day 7 Bile Canaliculi

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 24-well at 10x magnification

Example Culture Origin

Human Donor Demographics
  • Strain: Gottingen
  • Number of Donors: 3
  • Age(yrs): –
  • Gender: Male