HUREL® Mouse™

Co-culture comprised of cryopreserved primary mouse hepatocytes cultured with cells of non-parenchymal, stromal type.


Example Metabolic Activity (nmoles/hr/106 cells)

SubstrateEnzymeConcentration (µM)Day 1Day 4Day 8
7-EthoxycoumarinPhase I1000.0340.0170.016
7-HydroxycoumarinPhase II10051.546.50.21
7-HydroxycoumarinPhase II1001.761.020.08

Culture Condition and Morphology

Cryopreserved primary mouse hepatocytes were thawed and plated with HUREL PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to HUREL PlatinumHeps™ Basal Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/106 cells. All incubations were carried out in triplicate on days D1, D4, and D8 upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Col­lected supernatants were stored at -20°C until further LC/MS/MS analysis


Day 1 – Morphology

Day 7 – Morphology

Day 7 – Bile Canaliculi

Day 1 Morphology- Phase contrast image

Phase contrast image in a 24-well at a 10x magnification.

Day 7 Morphology-Phase contrast image

Phase contrast image in a 24-well at a 10x magnification.

Day 7 Bile Canaliculi assay

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (C-DCFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 96-well at 10x magnification with filters EX/EM 492-495/512-527 nm.


Example Culture Origin

Animal Donor Demographics
  • Strain: CD-1
  • Number of Donors: 5
  • Age(yrs)
  • Sex: Male