HUREL® Primate™

Co-culture comprised of cryopreserved primary primate hepatocytes cultured with cells of non-parenchymal, stromal type.

Example Metabolic Activity (nmoles/hr/106 cells)

SubstrateEnzymeConcentration (µM)Day 1Day 4Day 8
7-EthoxycoumarinPhase I (ECOD)1000.8530.2890.115
7-HydoxycoumarinPhase II (UGT)10078.3371.584.5
Phase II (SULT)8.688.5716.13

Culture Condition and Morphology

Cryopreserved cynomologus primate hepatocytes were thawed and plated with HUREL PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to HUREL PlatinumHeps™ maintenance Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/million cells. All incubations were carried out in triplicate on days 1, 4, and 8 (customer day) upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Collected supernatants were stored at -20°C until further LC/MS/MS analysis.

Day 1 – Morphology

Primate Day 1 Morphology

Phase contrast image in a 96-well at a 10x magnification

Day 7 – Morphology

Primate Day 7 Morphology

Phase contrast image in a 96-well at a 10x magnification

Day 7 – Bile Canaliculi

Primate Day 7 Bile Canaliculi

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 96-well at 10x magnification

Example Culture Origin

Animal Donor Demographics
  • Strain: Cynomolgus
  • Number of Donors: 3
  • Age (yrs): 2~3
  • Gender: Male